Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway. full article pdf download

Title of article - Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway.

Abstract

Purpose. In this report, we investigated the protective mechanism of scutellarin (SCU) in vitro and in vivo which could be involved in endothelial cGMP-dependent protein kinase (PKG), vasodilator stimulated phosphoprotein (VASP) pathway, and vascular endothelium dysfunction (EtD). Method. Human brain microvascular endothelial cells (HBMECs) with hypoxia reoxygenation (HR) treatment and rats with cerebral ischemia reperfusion (CIR) treatment were applied. Protein and mRNA expression of PKG, VASP, and p-VASP were evaluated by Western blot and RT-PCR methods. Vascular EtD was assessed by using wire myography to determine endothelium-dependent vasorelaxation in isolated rat basilar artery (BA). Result. In cultured HBMECs, SCU (0.1, 1, and 10 μM) increased cell viability, mRNA, protein level, and phosphorylative activity of PKG and VASP against HR injury. In HR model of BA, SCU increased protein level of P-VASP. In rat CIR model, wire myography demonstrated that SCU (45 and 90 mg/kg, i.v.) significantly reduced ischemic size by partially restoring the endothelium dependent vasodilation of BA; PKG inhibitor Rp-8-Br-cGMPS (50 μg/kg, i.v.) reversed this protection of SCU in CIR rats. Conclusion. SCU protects against cerebral vascular EtD through endothelial PKG pathway activation.

CLICK HERE for Full article

Click button above to go to original article link with complete article details for Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway.

Please CLICK HERE to discuss with us on facebook about this article.

Click below for Details of research Institute

School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, China ; Department of Respiratory Medicine, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.

Details of Journal for Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway.

Journal Title - Evidence-based complementary and alternative medicine : eCAM

ISSN - 1741-427X

Volume - 201

Issue - 0

Publish date - 2015-

Language - eng

Country - United States